The present invention relates to compositions and methods for providing RNA interference (RNAi) vectors comprising trigalactosyldiacylglycerol (tgd) biosynthesis enzyme constructs for increasing oil content of plants. Further, the use of tgd RNAi silencing vectors in combination with co-expression of heterologous oil regulating transcription factors, such as WRINKLED1, are contemplated to overcome the reduced growth and variable levels of embryonic lethality in plants with reduced TGD protein. Additionally, plants having reduced APS1, a gene encoding a major catalytic isoform of the small subunit of AGPase (AGP(-) plants), co-expressing a heterologous oil modulating transcription factor are contemplated for use in combination with plants having reduced TGD. Oil harvested from vegetative tissues of plants comprising vectors and genes of the present invention is contemplated for use in biofuel and biodiesel products.
 This invention was made with government support under Grant No. MCB0453858 by The National Science Foundation (NSF), USDA-CSREES grant number 2005-35504-16195 from the United States Department of Agriculture, and Grant No. DE_FG02-07ER20356 from the Department of Energy (DOE) and Great Lakes Bioenergy Research Center Cooperative Agreement DE-FC02-07ER64494 by the DOE. The government has certain rights in the invention.