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Engineering an Increase in Ethanol Production by Altering Cofactor Specificity

United States Patent Application

View the Complete Application at the US Patent & Trademark Office
The present invention provides for the manipulation of cofactor usage in a recombinant host cell to increase the formation of desirable products. In some embodiments, the invention provides for a recombinant microorganism comprising a mutation in one or more native enzymes such that their cofactor specificity is altered in such a way that overall cofactor usage in the cell is balanced for a specified pathway and there is an increase in a specific product formation within the cell. In some embodiments, endogenous enzymes are replaced by enzymes with an alternate cofactor specificity from a different species.
Lo, Jonathan (Hanover, NH), Guss, Adam M. (Knoxville, TN), Van Dijken, Johannes P. (Schiedam, NL), Shaw, IV, Arthur J. (Grantham, NH), Olson, Daniel G. (Norwich, VT), Herring, Christopher D. (Lebanon, NH)
14/ 361,948
November 30, 2012
STATEMENT REGARDING FEDERALLY-SPONSORED RESEARCH AND DEVELOPMENT [0001] This invention was funded, in part, by the United States government under a Department of Energy Biomass Program award #DE-FC36-07G017057. This invention was also funded, in part, by the BioEnergy Science Center (BESC) under the DOE Office of Science through award number DE-POS2-06ER64304. The U.S. Government has certain rights in this invention.