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United States Patent Application

View the Complete Application at the US Patent & Trademark Office
Los Alamos National Laboratory - Visit the Technology Transfer Division Website
Advances in sequencing technologies have dramatically reduced costs in producing high quality draft genomes. There are still many contigs and possible misassembled regions in those draft genomes. Described herein are methods for improving the quality of sequencing techniques, and particularly methods for overcoming the loading bias inherent in, for instance, the PacBio sequencing process. Compared to Sanger sequencing technology, the herein described method is not only cost-effective but also can close gaps greater than 2.5 Kb in a single round of reactions. It can also sequence through high GC regions and difficult secondary structures such as hairpin loops.
Zhang, Xiaojing (Los Alamos, NM), Davenport, Karen Walston (Los Alamos, NM), Green, Lance Duane (Jemez Springs, NM), Han, Shunsheng (Los Alamos, NM)
Los Alamos National Security, LLC
13/ 931,342
June 28, 2013
STATEMENT OF GOVERNMENT SUPPORT [0002] This invention was made with government support under Contract No. DEAC52-06NA25396 awarded by the U.S. Department of Energy. The government has certain rights in the invention.