Provided herein are methods of identifying a protein as soluble, as well as methods of identifying a soluble protein complex of at least two proteins. The methods allow for the determination of in vitro solubility, or both in vitro and in vivo solubility, of a protein or protein complex.
STATEMENT OF GOVERNMENT SUPPORT
 This invention was made with government support under Contract No. DE-AC52-06NA25396 awarded by the U.S. Department of Energy, and under the National Institutes of Health's Protein Structure Initiative, grant number 5U54GM074946-4. The government has certain rights in the invention.