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United States Patent Application

View the Complete Application at the US Patent & Trademark Office
Los Alamos National Laboratory - Visit the Technology Transfer Division Website
Disclosed herein are methods for producing compounds (such as 3,4-dihydroxybenzoate, catechol, cis,cis-muconate, or .beta.-carboxy-cis,cis-muconic acid) utilizing biosynthetic pathways in prokaryotic organisms expressing one or more heterologous genes. In some embodiments, the method includes expressing a heterologous asbF gene (for example, a gene having dehydroshikimate dehydratase activity) in a prokaryotic cell under conditions sufficient to produce the one or more compounds and purifying the compound. In additional embodiments, the method further includes expressing one or more of a heterologous 3,4-DHB decarboxylase gene, a heterologous catechol 1,2-dioxygenase gene, and a heterologous 3,4-DHB dioxygenase gene in the prokaryotic cell and purifying the compound.
Koppisch, Andrew Thomas (Flagstaff, AZ), Fox, David Thomas Shaw (Los Alamos, NM), Hotta, Kinya (Shizuoka, JP), Welsh, John D. (Pennington, NJ)
National University of Singapore (Singapore NM), Los Alamos National Laboratory, LC/IP (Los Alamos)
13/ 908,759
June 3, 2013
ACKNOWLEDGMENT OF GOVERNMENT SUPPORT [0002] This invention was made with government support under Contract No. DE-AC52-06NA25396 awarded by the U.S. Department of Energy. The government has certain rights in the invention.