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Methods of Introducing Nucleic Acids into Cellular DNA

United States Patent Application

20140045267
A1
View the Complete Application at the US Patent & Trademark Office
A method of introducing a nucleic acid sequence into a cell is provided where the cell has impaired or inhibited or disrupted primase activity or impaired or inhibited or disrupted helicase activity, or larger or increased gaps or distance between Okazaki fragments or lowered or reduced frequency of Okazaki fragment initiation, or the cell has increased single stranded DNA (ssDNA) on the lagging strand of the replication fork including transforming the cell through recombination with a nucleic acid oligomer. The present invention relates to methods of introducing one or more nucleic acid sequences into a cell having impaired or inhibited or disrupted primase activity or impaired or inhibited or disrupted helicase activity, or larger or increased gaps or distance between Okazaki fragments or lowered or reduced frequency of Okazaki fragment initiation.
LAJOIE, Marc J. (Cambridge, MA), GREGG, Christopher J. (Roslindale, MA), MOSBERG, Joshua A. (Cambridge, MA), CHURCH, George M. (Brookline, MA)
PRESIDENT AND FELLOWS OF HARVARD COLLEGE (Cambridge MA)
13/ 954,351
July 30, 2013
STATEMENT OF GOVERNMENT INTERESTS [0002] This invention was made with government support under Department of Energy Genomes to Life Center grant number DE-FG02-02ER63445 and National Institutes of Health grant number P50 HG005550. The Government has certain rights in the invention.