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Method for in vitro Recombination

United States Patent Application

View the Complete Application at the US Patent & Trademark Office
The present invention relates to an in vitro method, using isolated protein reagents, for joining two double-stranded (ds) DNA molecules of interest, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule share a region of sequence identity. The method allows the joining of a number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes. It can be used, e.g., to join synthetically produced sub-fragments of a gene or genome of interest.
Gibson, Daniel Glenn (Crofton, MD), Smith, Hamilton O. (Reisterstown, MD)
13/ 864,108
April 16, 2013
GRANT INFORMATION [0002] This invention was made with government support under Grant No. DE-FG02-02ER63453 awarded by the Department of Energy. The government has certain rights in the invention.