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NUCLEIC ACID DETECTION AND RELATED COMPOSITIONS METHODS AND SYSTEMS

United States Patent Application

20130210016
A1
View the Complete Application at the US Patent & Trademark Office
Lawrence Livermore National Laboratory - Visit the Industrial Partnerships Office Website
Provided herein are methods and systems for loop-mediated isothermal amplification of target polynucleotides on a sample without sample preparation. Methods and systems herein described also allow detection of cells and in particular bacterial cells on an untreated sample comprising the cells, and allow in some embodiments specific detection of bacterial cells such as B. anthracis.
DUGAN, Lawrence (Modesto, CA), Souza, Brian E. (Livermore, CA), Bearinger, Jane P. (Berwyn, PA)
Lawrence Livermore National Security, LLC (Livermore CA)
13/ 767,726
February 14, 2013
STATEMENT OF GOVERNMENT GRANT [0002] The United States Government has rights in this invention pursuant to Contract No. DE-AC52-07NA27344 between the U.S. Department of Energy and Lawrence Livermore National Security, LLC, for the operation of Lawrence Livermore National Security.