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RECOMBINANT RENEWABLE POLYCLONAL ANTIBODIES

United States Patent Application

20130085072
A1
View the Complete Application at the US Patent & Trademark Office
Los Alamos National Laboratory - Visit the Technology Transfer Division Website
Described herein are methods that combine phage and yeast display to create polyclonal antibodies that are renewable, and when amplified over 100 million fold, maintain diversity without loss of representation of any of the antibodies present. The antibody representation remains essentially constant, as confirmed by deep sequencing. The provided methods allow generation, use and propagation of polyclonal antibodies, without concern that representation is lost. Furthermore, because the derivation of the polyclonal pool is carried out in vitro using phage and yeast display, it is possible in various embodiments to eliminate reactivities that are considered undesirable. Additionally, the polyclonal pool can be enriched for higher affinity antibodies.
Bradbury, Andrew M. (Santa Fe, NM), Kiss, Csaba (Los Alamos, NM), D'Angelo, Sara (Los Alamos, NM), Ferrara, Fortunato (Los Alamos, NM), Naranjo, Leslie A. (Los Alamos, NM), Gaiotto, Tiziano (Los Alamos, NM)
LOS ALAMOS NATIONAL SECURITY, LLC (Los Alamos NM)
13/ 631,331
September 28, 2012
ACKNOWLEDGMENT OF GOVERNMENT SUPPORT [0002] This invention was made with U.S. government support under Contract No. DE-AC52-06NA25396 awarded by the U.S. Department of Energy. The U.S. government has certain rights in the invention.