Disclosed herein are Split-Fluorescent proteins (SFPs) including Split-Yellow Fluorescent Proteins and Split-Cyan Fluorescent proteins. Further disclosed are methods of using SFPs. For example, methods of identifying the subcellular localization of a protein and methods of identifying the membrane topology of a membrane protein are disclosed herein.
STATEMENT OF GOVERNMENT SUPPORT
 This invention was made with government support under Contract No. DE-AC52-06NA25396 awarded by the U.S. Department of Energy, and under the National Institutes of Health's Protein Structure Initiative, grant number 5U54GM074946-4. The government has certain rights in the invention