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ENGINEERED MICROOGRANISMS CAPABLE OF PRODUCING TARGET COMPOUNDS UNDER ANAEROBIC CONDITIONS

United States Patent Application

20120190089
A1
View the Complete Application at the US Patent & Trademark Office
The present invention is generally provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention.
Buelter, Thomas (Denver, CO), Meinhold, Peter (Denver, CO), Feldman, Reid M. Renny (San Francisco, CA), Hawkins, Andrew C. (Parker, CO), Bastian, Sabine (Pasadena, CA), Arnold, Frances (La Canada, CA), Urano, Jun (Aurora, CA)
California Institute of Technology (Pasadena CA), Gevo, Inc. (Englewood CO)
13/ 303,884
November 23, 2011
STATEMENT REGARDING FEDERALLY FUNDED RESEARCH [0002] This invention was made with government support under contract DE-FG02-07ER84893, awarded by the Department of Energy, and contract W911NF-09-2-0022, awarded by the United States Army Research Laboratory. The government has certain rights in the invention.