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APPARATUS AND METHODS FOR HIGH THROUGHPUT BIOMOLECULE SEPARATION AND ANALYSIS

United States Patent Application

20120043208
A1
View the Complete Application at the US Patent & Trademark Office
Lawrence Berkeley National Laboratory - Visit the Technology Transfer and Intellectual Property Management Department Website
A multi-channel gel electrophoresis apparatus for efficiently collecting molecules isolated by gel electrophoresis so they can be further analyzed, identified, or used as reagents or medications. The apparatus using a novel "tagless" strategy that combines multi-dimensional separation of endogenous complexes with mass spectrometric monitoring of their composition. In this procedure, putative protein complexes are identified based on the co-migration of collections of polypeptides through multiple orthogonal separation steps. A majority of E. coli proteins are shown to remain in stable complexes during fractionation of a crude extract through three chromatographic steps.
Jin, Jian (Berkeley, CA), Biggin, Mark D. (Berkeley, CA), Nordmeyer, Robert A. (San Leandro, CA), Dong, Ming (El Cerrito, CA), Cornell, Earl W. (Antioch, CA), Choi, Megan (El Cerrito, CA), Witkowski, Halina Ewa (Walnut Creek, CA), Han, Bong-Gyoon (Castro Valley, CA), Glaeser, Robert M. (Berkeley, CA)
The Regents of the University of California (Oakland CA)
13/ 176,704
July 5, 2011
STATEMENT OF GOVERNMENTAL SUPPORT [0002] This invention was made during work supported under Contract No. DE-AC02-05CH11231 awarded by the U.S. Department of Energy. The government has certain rights in this invention.