Improved systems and methods for reducing costs and increasing yields of cellulosic ethanol are disclosed herein, along with plants genetically transformed for increased biomass, expression of lignocellulolytic enzyme polypeptides, and/or simplification of harvesting and downstream processing. Methods for processing biomass from these transgenic plants that involve less severe and/or less expensive pre-treatment protocols than are typically employed are also disclosed.
 This invention was made with U.S. government support under the United States Department of Agriculture and Department of Energy Biomass Grant No. DE-PS36-06G096002F to Edenspace Systems Corporation and contract No. DE-AC36-08G028308 between the United States Department of Energy and the Alliance for Sustainable Energy, LLC, manager and operator of the National Renewable Energy Laboratory. The government of the United States of America has certain rights in the invention.