Nano-microfluidic devices and uses thereof are described. In particular, systems and methods are described for continuous real-time monitoring and analysis of targets in thin liquid films; such targets can include living cells and tissues. In some embodiments, nano-microfluidic devices can be utilized to observe living cells in layers of thin liquid media by IR-spectroscopy.
STATEMENT REGARDING FEDERALLY SPONSORED R&D
 This invention was made with government support under Contract DE-AC02-05CH11231 and W-7405-ENG-48 awarded by the U.S. Department of Energy, and under Contract No. DE-AC52-07NA27344 awarded by the U.S. Department of Energy and the NNSA. The government has certain rights in this invention.