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IN VIVO INCORPORATION OF AN UNNATURAL AMINO ACID COMPRISING A 1,2-AMINOTHIOL GROUP

United States Patent Application

20110076718
A1
View the Complete Application at the US Patent & Trademark Office
The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate unnatural amino acids that comprise a 1,2 aminothiol group into polypeptides. The invention provides translation systems in which polypeptides comprising unnatural amino acids that comprise a 1,2 aminothiol group can be produced. The invention also provides methods for producing polypeptides containing unnatural amino acids that comprise a 1,2 aminothiol group. Also provided by the invention are compositions comprising orthogonal aminoacyl-tRNA synthetases that preferentially aminoacylate a cognate orthogonal tRNA with unnatural amino acids that comprise a 1,2 aminothiol group. The invention provides methods for the synthesis of the unnatural amino acid 2-amino-3-(4-(2-amino-3-mercaptopropan-amido)phenyl)-propanoic acid.
Ficht, Simon (San Diego, CA), Jahnz, Michael (Berlin, DE), Grunewald, Jan (San Diego, CA), Schiller, Stefan (Pfaffenweiler, DE), Schultz, Peter G. (La Jollla, CA)
The Scripps Research Institute
12/ 735,966
February 27, 2009
STATEMENT AS TO RIGHTS TO INVENTIONS MADE UNDER FEDERALLY SPONSORED RESEARCH AND DEVELOPMENT [0002] The invention was made with United States Government support under Grant DE-FG02-03ER46051 from the Department of Energy. The United States Government has certain rights in the invention.