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IN VIVO EVOLUTION OF HYDROGENASES USING A HYDROGEN-SENSING SYSTEM

United States Patent Application

20110059863
A1
View the Complete Application at the US Patent & Trademark Office
National Renewable Energy Laboratory - Visit the NREL Technology Transfer Website
Provided herein are methods for measuring H2 production by a hydrogenase. Also provided are methods for evolving a hydrogenase or hydrogen-sensing system by comparing the level of production of H2 by the hydrogenase to the level Of H2 produced by a wild-type hydrogenase cultured under the same conditions and selecting a microorganism with increased H2 production over the H2 production by a microorganism having a wild-type hydrogenase. Further provided herein are microorganisms and plasmids comprising a hupSL promoter coupled with a reporter gene.
Ghirardi, Maria Lucia (Lakewoood, CO), Wecker, Matt (Boulder, CO), Posewitz, Matthew (Golden, CO)
Alliance for Sustainable Energy, LLC (Golden CO)
12/ 918,182
March 10, 2009
CONTRACTUAL ORIGIN [0001] The United States Government has rights in this invention under Contract No. DE-AC36-08G028308 between the United States Department of Energy and the Alliance for Sustainable Energy, LLC, manager and operator of the National Renewable Energy Laboratory.