A strategy for enzymatic biomass conversion that involves enzyme recovery and reuse. Enzyme recovery is achieved by attaching enzymes to paramagnetic nanoparticles. By magnetically attracting the paramagnetic nanoparticles, enzymes may be recovered for reuse. The process of enzymatic biomass hydrolysis includes: attaching biomass degrading enzymes to a surface of functionalized particles having paramagnetic properties; mixing the particles with a biomass; recovering the enzymes via magnetic separation; and re-using the enzymes in a subsequent process. The process provides a product feed stream which may be subjected to further processing. The process may be repeated multiple times wherein the biomass is sequentially subjected to different enzymes.
STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT
 This invention was made with government support under grant number DE-FG02-96ER20215 awarded by the Department of Energy. The government has certain rights in the invention.