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Process for conjugating biomolecules to hydrophobic membrane-incorporated molecules

United States Patent Application

20040171175
A1
View the Complete Application at the US Patent & Trademark Office
Los Alamos National Laboratory - Visit the Technology Transfer Division Website
A process is provided of conjugating a recognition element such as a biomolecule to a hydrophobic multifunctional linker molecule by incorporating a multifunctional linker molecule including one or more anchoring groups, a reporter group and a reactive site thereon into a membrane, and, reacting the membrane including the incorporated multifunctional linker molecule with a pre-selected recognition element to form a covalently bound recognition element-multifunctional linker molecule-membrane assembly. Also, a chemical assembly suitable for subsequent covalent attachment of a recognition element is provided such assembly including a multifunctional linker molecule including one or more anchoring groups, a reporter group, and a hydrophilic spacer terminated by a reactive group capable of subsequent covalent bonding, the one or more anchoring groups incorporated in a membrane.
Swanson, Basil I. (Los Alamos, NM), Unkefer, Clifford J. (Los Alamos, NM), Schmidt, Jurgen G. (Los Alamos, NM), Reed, Scott M. (Tesuque, NM), Shively, John E. (Arcadia, CA), Li, Lin (Monrovia, CA)
10/ 377,515
February 28, 2003
[0001] This invention was made with government support under Contract No. W-7405-ENG-36 awarded by the U.S. Department of Energy. The government has certain rights in the invention.