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Method for rapid identification and quantification of microorganisms

United States Patent Application

View the Complete Application at the US Patent & Trademark Office
Los Alamos National Laboratory - Visit the Technology Transfer Division Website
Method for unamplified, selective identification of a microorganism comprising obtaining a sample comprising the microorganism, wherein the sample comprises unseparated genetic material of the microorganism; adding to the sample a molecular beacon comprising an nucleic sequence which is complementary to at least one nucleic acid sequence in the microorganism; and heating the sample; wherein the molecular beacon hybridizes with said nucleic acid sequence to produce a detectable signal.
Creek, Kathryn L. (Seattle, WA), Castro, Alonso (Santa Fe, NM), Gray, Perry C. (Los Alamos, NM)
The Regents of the University of California
12/ 284,632
September 23, 2008
STATEMENT OF FEDERAL RIGHTS [0001] The United States government has rights in this invention pursuant to Contract No. DE-AC52-06NA25396 between the United States Department of Energy and Los Alamos National Security, LLC for the operation of Los Alamos National Laboratory.