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Constructing very long DNA sequences from synthetic DNA molecules

United States Patent Application

20030180781
A1
View the Complete Application at the US Patent & Trademark Office
Lawrence Livermore National Laboratory - Visit the Industrial Partnerships Office Website
A method of fabricating a DNA molecule of user-defined sequence. A multiplicity of DNA sequence segments that will comprise the DNA molecule of user-defined sequence are preselected using computational techniques to break said user-defined sequence into fragments of defined size. The multiplicity of DNA sequence segments are combined with at least one polymerase enzyme. The multiplicity of DNA sequence segments join to produce the DNA molecule of user-defined sequence.
Christian, Allen T. (Tracy, CA), Mariella, Raymond P. JR. (Danville, CA), Williams, Jennifer M. (Pleasanton, CA)
The Regents of the University of California
10/ 394,337
March 19, 2003
[0002] The United States Government has rights in this invention pursuant to Contract No. W-7405-ENG-48 between the United States Department of Energy and the University of California for the operation of Lawrence Livermore National Laboratory.